- Both PCR and DNA Sequencing start in the the test tubes!
- DNA polymerization is involved in both processes.
Differences
PCR
- DNA denatures at high temperature (around 94-96 C)
- Special DNA polymerase that can withstand heat called taq polymerase is used
- Primers are used to initiate elongation
DNA Sequencing
- Dideoxyribo-nucleotides are used, which terminates the sequence since they have no OH group to bind a proceeding nucleotide.
- Only a very small amount of ddNTP is used so that incomplete "digestion" occurs, thus each possible termination site (complementary nucleotide to the ddNTP) is shown
This is very acknowledged post. You have described clear and correct about PCR and DNA similarities & differences.
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